Methylation-modified peptides, inozivikanwawo semethylation-recognized peptides, iprotein post-translational decorations (PTMs) uye inoita basa rakakosha rekutonga mune zvingangoita zvese zviitiko zvehupenyu mumaseru.Mapuroteni anogadziriswa nemethyltransferase kuendesa mapoka ehydroxyl kune mamwe maamino acid akasara kune covalent kusunga.Methylation inzira inodzoreredzwa yekuchinja inogadziriswa ne demethylases.
Zvidzidzo zvakaratidza kuti yakajairika methylated/demethylated amino acids kazhinji lysine (Lys) uye arginine (Arg).Zvidzidzo zvakawana kuti histone lysine methylation ine mabasa akasiyana-siyana ehupenyu akadai se stem cell kuchengetedza uye kupatsanura, X chromosome inactivation, transcription regulation uye DNA kukuvara kwemhinduro.", kazhinji inokanganisa chromatin condensation uye inodzvinyirira gene expression."Histone arginine methylation inobata basa rakakosha mukugadzirisa kunyorwa kwemajini uye inogona kukanganisa maitiro akasiyana-siyana emuviri mumaseru, kusanganisira kugadzirisa kweDNA, kutapurirana kwechiratidzo, kukura kwesero, uye carcinogenesis.Naizvozvo, Guopeptide Biology yakanyatso gadzira tekinoroji yemethyl yekushongedza peptides, iyo inogadziridzwa nesainzi mushure mekushandurwa kweprotein (PTMS) kubatsira mukutsvagisa.
Kugadziriswa kweMethylation (Me1, Me2, Me3)
Yemhando yepamusoro Fmoc-Ls(Ini,Boc)-OH, Fmoc-Lys(Me2)-OH, Fmoc-Lys(Me3)-OH.HCL, Fmoc-Arg(Me,Pbf)-OH, Fmoc-Arg(Me) 2-OH.HCl(asymmetrical), F yakashandiswa moc-Arg(me)2-OH.HCl(symmetrical) uye zvimwe zvinhu zvakagadzirwa neFOC solid-phase synthesis process kuti vawane Lys and Arg methylated peptides, uye zvigadzirwa. dzakacheneswa neHPLC.Yakakodzera misa spectra, HPLC chromatograms uye COA inopihwa kune yapera chigadzirwa.
Nguva yekutumira: Nov-24-2023