Peptide-senge synthesis tekinoroji
Tsvagiridzo nekuvandudzwa kwemishonga yepeptide iri kukura nekukurumidza mukurapa.Nekudaro, kugadzirwa kwemishonga yepeptide kunogumira nehunhu hwavo.Semuenzaniso, nekuda kwekunzwa kwakakosha kune enzymatic hydrolysis, kugadzikana kunodzikiswa, uye kusiyanisa kweiyo steric conformation inokonzeresa yakaderera yakanangana, yakaderera hydrophobicity, uye kushaikwa kweiyo chaiyo nzira yekufambisa.Kuti ukunde mapeptide aya, mhinduro dzakawanda uye kubudirira kweimwe mhando yepeptide ndeimwe yacho.
Rudzi rwepeptide (zita rechirungu: Peptoid) kana Poly - N - pachinzvimbo cheglycine (zita reChirungu: Poly real - N - substitutedglycine), ndeye quasi peptide makomisheni epeptide mumaketani makuru.Iyo alpha carbon side cheni inotamisa iyo main cheni nitrogen panzvimbo pecheni yepadivi.Mupolypeptide yepakutanga, boka reR reamino acid parutivi cheni rinomiririra makumi maviri emaamino acids akasiyana, asi boka reR rine zvimwe zvingasarudzwa mupeptoid.Mupeptide, peptide pacheni huru yeamino acids mualpha kabhoni nitrogen pachinzvimbo chekuendesa necheni yepadivi kune cheni huru.Zvakakodzera kutaura kuti peptides kazhinji haaburitse maumbirwo akaenzana epamusoro-soro sezvikamu zvechipiri mumapeptide nemapuroteni nekuda kwekushaikwa kwehydrogen kumusana wenitrogen.Peptide yekutanga chinangwa ndechekugadzira yakagadzikana uye protease peptide yediki molecule mishonga.
Kuongororwa kwepeptide-senge synthesis matekiniki
Nzira ye peptide synthesis yakaunzwa
Iyo inowanzozivikanwa peptide-senge synthesis nzira ndiyo subsingle synthesis nzira yakagadzirwa naRonZuckermann, imwe neimwe yakakamurwa kuita nhanho mbiri: acylation uye kutamiswa.Muacylation, danho rekutanga ndere kumisa haloacetic acid kuti iite nemaamine akasara pakupera kwedanho rekutanga, kazhinji diisopropyl carbonized diimine.bromoacetic acid yakashandiswa ne diisopropylcarbodiimide."Mukutsiva maitiro (bimolecular nucleophilic substitution reactions), amine, kazhinji yekutanga, inorwisa imwe halogen kuumba N-inotsiva glycine."Iyo subunitary synthetic nzira inoshandisa inowanikwa zviri nyore maamines kugadzira peptides, nekudaro ichigonesa makemikari synthesis yepeptides.
Kuwedzera kwakasimba mukirasi peptides synthesis ine ruzivo rwakapfuma, inogona kukupa iwe akasiyana emhando yepeptide synthesis sevhisi.
Kuongororwa kwepeptide-senge synthesis matekiniki
Kubatsira kwepeptide yakadaro
Yakanyanya kugadzikana: peptoids yakagadzikana mu vivo kupfuura peptides.
More selectivity: Peptoids yakanyatsokodzera kusanganisirwa zvidzidzo zvekuwanikwa kwezvinodhaka nekuti yakakura siyana yemapolypeptide ekuvaka zvidhinha inogona kuwanikwa nekugadziriswa kweiyo musana amino boka.
Kunyanya kushanda: Kuwanda kwezvimiro zvepeptoid kunogona kuita peptoid sarudzo yakanaka yekuongorora nzira yekukurumidza kuwana chaiwo maumbirwo anosunga kumapuroteni.
Kuwanda kwemusika kugona: hunhu hwemhando yepeptide ngaive mhando yekugadzirwa kwezvinodhaka ine mukana mukuru.
Nguva yekutumira: Zvita-07-2023