Phosphorylation inokanganisa zvikamu zvose zvehupenyu hwemaserura, uye protein kinases inobata zvikamu zvose zvekushanda kwekukurukurirana kwemukati nekugadzirisa nzira dzechiratidzo uye maitiro emagetsi.Zvisinei, aberrant phosphorylation zvakare inokonzera zvirwere zvakawanda;kunyanya, mutated protein kinases uye phosphatases inogona kukonzera zvirwere zvakawanda, uye dzakawanda zvechisikigo chepfu uye hutachiona hunewo mhedzisiro kuburikidza nekuchinja phosphorylation mamiriro eintracellular mapuroteni.
Phosphorylation ye serine (Ser), threonine (Thr), uye tyrosine (Tyr) inodzokororwa mapuroteni ekugadzirisa maitiro.Ivo vanobatanidzwa mukutonga kweakawanda ma cellular zviitiko, senge receptor siginecha, protein kushamwaridzana uye segmentation, activation kana inhibition yeprotein basa, uye kunyangwe sero kupona.Phosphates inopomerwa zvisina kunaka (mbiri mbiri dzisina kunaka paboka rephosphate).Nokudaro, kuwedzera kwavo kunoshandura zvinhu zveprotein, izvo zvinowanzochinja, zvichiita kuti kuchinja kwemaitiro eprotini.Kana boka rephosphate rabviswa, kuumbwa kweprotein kunodzokera kumamiriro ayo epakutanga.Kana iwo maviri mapuroteni ekugadzirisa achiratidza zviitiko zvakasiyana, phosphorylation inogona kuita senge molecular switch yeprotein kuti idzore kuita kwayo.
Mahomoni akawanda anogadzirisa basa rema enzymes chaiwo nekuwedzera phosphorylation state ye serine (Ser) kana threonine (Thr) zvakasara, uye tyrosine (Tyr) phosphorylation inogona kukonzerwa nekukura zvinhu (zvakadai se insulini).Mapoka efosfate emaamino acids aya anogona kukurumidza kubviswa.Saka, Ser, Thr, uye Tyr inoshanda semamolecular switch mukugadzirisa kwezviitwa zvemaserura senge bundu rinowedzera.
Synthetic peptides inoita basa rinobatsira zvikuru mukudzidza kweprotein kinase substrates nekudyidzana.Nekudaro, pane zvimwe zvinhu zvinotadzisa kana kudzikisira kuchinjika kwephosphopeptide synthesis tekinoroji, sekutadza kuwana yakazara otomatiki yakasimba-chikamu synthesis uye kushomeka kwekubatana kuri nyore nemapuratifomu ekuongorora.
Iyo papuratifomu yakavakirwa peptide synthesis uye phosphorylation gadziriso tekinoroji inokunda zvipimo izvi uku ichivandudza synthesis kunyatsoshanda uye scalability, uye chikuva chakanyatsokodzera kudzidza kweprotein kinase substrates, maantigen, anosunga mamorekuru, uye inhibitors.
Nguva yekutumira: May-31-2023