Sechiridzwa chepamusoro-chaizvo, HPLC inogona kutungamira nyore kune mamwe matambudziko madiki anonetsa kana ikasashandiswa nenzira kwayo panguva yekushandiswa.Imwe yematambudziko akajairika idambudziko rekoramu yekumanikidza.Maitiro ekukurumidza kugadzirisa dambudziko rechromatograph.Iyo HPLC system inonyanya kuve nebhodhoro rekuchengetedza, pombi, jekiseni, koramu, kamuri yekupisa tembiricha, detector uye data processing system.Kune iyo yose hurongwa, mbiru, mapombi uye detectors ndizvo zvakakosha zvikamu uye nzvimbo huru dzinotarisana nezvinetso.
Kiyi yekumanikidza koramu inzvimbo inoda kunyatsotariswa kana uchishandisa HPLC.Iko kugadzikana kwekoramu kudzvanywa kwakabatana zvakanyanya nechromatographic peak chimiro, kushanda kwekoramu, kupatsanurwa kwakanaka uye nguva yekuchengetedza.Column pressure stability hazvireve kuti kudzvinyirirwa kwacho kwakadzikama pamutengo wakagadzikana, asi kuti kudzvinyirirwa kwacho kuri pakati pe345kPa kana 50PSI (inobvumira kushandiswa kwe gradient elution kana mbiru yekumanikidza yakagadzikana uye zvishoma nezvishoma kuchinja).Kunyanya kukwira kana kuderera zvakanyanya idambudziko remakoramu.
Iyo inonyanya kutarisana neHPLC kukundikana uye mhinduro
1, high pressure ndiyo inonyanya kunetsa mukushandiswa kweHPLC.Izvi zvinoreva kusimuka kamwe kamwe mukumanikidza.Kazhinji, pane zvikonzero zvinotevera: (1) Kazhinji, izvi zvinokonzerwa nekuyerera kwegero blockage.Panguva ino, tinofanira kuiongorora.a.Chekutanga, cheka kupinza kwepombi yevacuum.Panguva iyi, chubhu yePEEK yakazadzwa nemvura zvekuti chubhu yePEEK yaive idiki pane bhodhoro rekunyungudutsa kuti vaone kana mvura ichidonha pamadiro.Kana iyo mvura isiri kudonha kana kudonha zvishoma nezvishoma, iyo solvent sefa musoro unovharika.Kurapa: Nyura mu30% nitric acid kwehafu yeawa uye sukurudza nemvura ine ultraure.Kana mvura ikadonha zvisina tsarukano, musoro wesefa wesolvent wakajairika uye uri kuongororwa;b.Vhura iyo Purge valve kuitira kuti nharembozha isapfuure nepakati, uye kana kudzvanywa kusinganyanyi kuderedzwa, iyo chena chena musoro yakavharwa.Kurapa: Machena machena akacheneswa akabviswa uye sonicated ne10% isopropanol kwehafu yeawa.Tichifunga kuti dzvinyiriro inodonha pasi pe100PSI, musoro wakachena wakasefa wakajairika uye uri kuongororwa;c.Bvisa magumo ekubuda kwekoramu, kana kudzvinyirira kusingaderedze, iyo column inovharwa.Kurapa: Kana iri buffer salt blockage, sukurudza 95% kusvika kumanikidza kwakajairika.Kana chipingamupinyi chacho chikakonzerwa nechimwe chinhu chakachengetedzwa zvakanyanya, kuyerera kwakasimba kupfuura yazvino nharembozha kunofanirwa kushandiswa kumhanyisa kune yakajairika kudzvanywa.Kana iyo yenguva refu yekuchenesa dzvinyiriro isingadzike maererano neiyo nzira iri pamusoro, iyo inopinza uye yekubuda kwekoramu inogona kutariswa seyakabatana kune chiridzwa pane zvinopesana, uye iyo column inogona kucheneswa ne mobile phase.Panguva ino, kana iyo mbiru yekumanikidza ichiri isina kuderedzwa, iyo mbiru yekupinda sieve ndiro inogona kungotsiviwa, asi kana oparesheni isina kunaka, zviri nyore kutungamira mukuderedzwa kwekoramu maitiro, saka edza kushandisa zvishoma.Nokuda kwezvinetso zvakaoma, kuchinja kwekoramu kunogona kufungwa.
(2) Kusarurama kweyero yekuyerera: Yakarurama yekuyerera mwero inogona kusetwa patsva.
(3) Kuyerera kusingakodzeri kuyerera: iyo viscosity index yezvikamu zvakasiyana zvekuyerera zvakasiyana, uye inoenderana system inomanikidza yekuyerera neakakwira viscosity zvakare yakakura.Kana zvichikwanisika, yakaderera viscosity solvents inogona kutsiviwa kana kugadzika zvakare uye yakagadzirirwa.
(4) System kudzvanywa zero Drift: gadzirisa zero yemvura level sensor.
2, kudzvanywa kwakadzikira (1) kunowanzo kukonzerwa nekubuda kwehurongwa.Zvekuita: Tsvaga imwe neimwe yekubatanidza, kunyanya iyo interface kumagumo ese ekoramu, uye simbisa nzvimbo inovuza.Bvisa posvo uye simbisa kana mutsara firimu rePTFE nesimba rakakodzera.
(2) Iyo gasi inopinda pombi, asi kudzvinyirira kunowanzo kusagadzikana panguva ino, yakakwirira uye yakaderera.Zvakanyanya kukosha, pombi haizokwanisi kutora mvura.Nzira yekurapa: vhura valve yekuchenesa uye uchenese pamwero wekuyerera we3 ~ 5ml / min.Kana zvisina kudaro, mabhuru emhepo aitsvagwa pavharuvhu yekubudisa simba achishandisa chubhu yakatsaurirwa yetsono.
(3) Hapana mobile phase inobuda: tarisa kana paine chikamu chinofambiswa mubhodhoro remvura, kana singi yakanyudzwa muchikamu chekufambisa, uye kana pombi iri kushanda.
(4) Ivhavha yekutaridzirwa haina kuvharwa: vheji yereferensi yakavharwa mushure mekuderera.Kazhinji inodzika kusvika ku0.1.~ 0.2mL/ min mushure mekuvhara vheji yereferensi.
Pfupiso:
Mune ino bepa, matambudziko akajairika chete mune yemvura chromatography anoongororwa.Chokwadika, mukushandisa kwedu kunoshanda, tichasangana nezvimwe zvinetso zvakawanda.Mukubata kukanganisa, tinofanira kutevera mitemo inotevera: kungoshandura chinhu chimwe chete pane imwe nguva kuti tione hukama huri pakati pekufungidzira uye dambudziko;Kazhinji, kana tichitsiva zvikamu zvekugadzirisa matambudziko, tinofanira kungwarira kudzorera zvikamu zvakabviswa zvisina kusimba kumashure kudzivirira tsvina;Kugadzira tsika yakanaka yekurekodha ndiyo kiyi yekubudirira kwekukanganisa kubata.Mukupedzisa, kana uchishandisa HPLC, zvakakosha kuterera kune sampuli pretreatment uye kushanda kwakaringana uye kugadzirisa kwemidziyo.
Nguva yekutumira: Sep-18-2023