Nzira yekubvisa
Mumakore ekuma1950 nema1960, nyika zhinji pasi rose, kusanganisira China, dzainyanya kubvisa peptides kubva munhengo dzemhuka.Semuenzaniso, jekiseni re thymosin rinogadzirwa nekuuraya mhuru ichangoberekwa, kubvisa thymus yayo, uye ipapo kushandisa oscillating separation biotechnology kuparadzanisa peptides kubva mhuru thymus.Iyi thymosin inoshandiswa zvakanyanya kudzora nekusimudzira masero ekudzivirira emuviri muvanhu.
Natural bioactive peptides inoparadzirwa zvakanyanya.Kune akawanda bioactive peptides mumhuka, zvidyarwa uye Marine zvipenyu mumasikirwo, ayo anotamba akasiyana siyana emuviri mabasa uye anochengetedza akajairika hupenyu zviitiko.Aya echisikigo bioactive peptides anosanganisira echipiri metabolites ezvipenyu zvakaita semishonga inorwisa mabhakitiriya nemahomoni, pamwe ne bioactive peptides iripo mune dzakasiyana siyana masisitimu.
Parizvino, akawanda bioactive peptides akaparadzaniswa nevanhu, mhuka, chirimwa, microbial uye Marine zvipenyu.Nekudaro, bioactive peptides anowanzo kuwanikwa muhuwandu hwakaderera muzvipenyu, uye hunyanzvi hwazvino hwekuparadzanisa uye kuchenesa bioactive peptides kubva kuzvisikwa zvipenyu haina kukwana, ine mutengo wakakwira uye yakaderera bioactivity.
Iyo inowanzoshandiswa nzira dzekubvisa peptide uye kuparadzanisa dzinosanganisira salting kunze, ultrafiltration, gel filtration, isoelectric point precipitation, ion exchange chromatography, affinity chromatography, adsorption chromatography, gel electrophoresis, nezvimwewo.
Acid-base nzira
Acid uye alkali hydrolysis inonyanya kushandiswa mukuyedza masangano, asi haiwanzo kushandiswa mukugadzira maitiro.Mukuita kwe alkaline hydrolysis yemapuroteni, akawanda amino acids akadai serine uye threonine anoparadzwa, racemization inoitika, uye nhamba huru yezvikafu inorasika.Nokudaro, nzira iyi haiwanzoshandiswa mukugadzirwa.Acid hydrolysis yemapuroteni haikonzeri racemization yeamino acids, hydrolysis inokurumidza uye maitiro akazara.Nekudaro, izvo zvisingabatsiri tekinoroji yakaoma, kutonga kwakaoma uye kusvibiswa kwakakomba kwezvakatipoteredza.Iyo molecular huremu kugovera kwepeptides haina kuenzana uye haina kugadzikana, uye mabasa avo emuviri akaoma kuona.
Enzymatic hydrolysis
Mazhinji bioactive peptides anowanikwa mumaketani marefu emapuroteni munzvimbo isingashande.Kana yakaitwa hydrolyzed neprotease chaiyo, peptide yavo inoshanda inoburitswa kubva mukutevedzana kweamino kweprotein.Enzymatic kutorwa kwe bioactive peptides kubva kumhuka, zvidyarwa uye Marine zvipenyu yanga iri yekutarisisa kwekutsvaga mumakumi emakore apfuura.
Enzymatic hydrolysis ye bioactive peptides ndiko kusarudzwa kweproteases akakodzera, kushandisa mapuroteni se substrates uye hydrolyzing mapuroteni kuwana nhamba huru yebioactive peptides ine mabasa akasiyana-siyana emuviri.Mukugadzira maitiro, tembiricha, PH kukosha, enzyme concentration, substrate concentration uye zvimwe zvinhu zvine hukama ne enzymatic hydrolysis maitiro ediki peptides, uye kiyi isarudzo ye enzyme.Nekuda kwema enzymes akasiyana anoshandiswa enzymatic hydrolysis, kusarudzwa uye kugadzirwa kwema enzymes, uye akasiyana mapuroteni masosi, mapeptide anozobuda anosiyana zvakanyanya muhukuru, kugoverwa kwehuremu, uye kuumbwa kweamino acid.Mumwe anowanzosarudza maprotease emhuka, akadai sepepsin uye trypsin, uye maprotease emiti, akadai sebromelain uye papain.Nekuvandudzwa kwesainzi uye tekinoroji uye nekuenderera mberi kwekuvandudzwa kweiyo biological enzyme tekinoroji, akawanda uye akawanda ma enzymes achawanikwa nekushandiswa.Enzymatic hydrolysis yakashandiswa zvakanyanya mukugadzirira bioactive peptides nekuda kwehunyanzvi hwayo hwekuita uye kuderera kwekudyara.
Nguva yekutumira: May-30-2023