Mukutsvaga kwebiology, polypeptides ine kutevedzana kwakareba inowanzoshandiswa.Kune mapeptides ane anopfuura makumi matanhatu amino acids mukutevedzana, gene expression uye SDS-PAGE anowanzo shandiswa kuwana.Nekudaro, iyi nzira inotora nguva yakareba uye yekupedzisira yekuparadzanisa chigadzirwa haina kunaka.
Matambudziko uye mhinduro dzehurefu peptide synthesis
Mukubatanidzwa kwepeptides refu, isu tinogara takatarisana nedambudziko, ndiko kuti, steric chipingamupinyi che condensation reaction inowedzera nekuwedzera kwekutevedzana mune synthesis, uye nguva yekuita inoda kugadziridzwa kuita kuti mhinduro ikwane.Nekudaro, iyo yakareba nguva yekuita, iyo yakawanda mhedzisiro inogadzirwa, uye chikamu chechinangwa peptide chinoumbwa.Masara akadaro - akashomeka peptide cheni ndiyo yakakosha tsvina inogadzirwa mune refu peptide synthesis.Naizvozvo, mukuumbwa kwepeptide refu, dambudziko rakakosha ratinofanira kukunda ndere kuongorora mamiriro emhando yepamusoro maitiro uye maitiro ekuita, kuitira kuti amino acid condensation reaction iwedzere uye izere.Mukuwedzera, kuderedza nguva yekuita, nokuti iyo yakareba nguva yekuita, iyo yakawanda isingadzoreki maitiro emativi, zvakanyanya kuoma ne-zvigadzirwa.Naizvozvo, pfungwa nhatu dzinotevera dzinopfupikiswa:
Microwave synthesis inogona kushandiswa: Kune mamwe maamino acids anosangana mukugadzirisa maitiro ayo asiri nyore kubatanidzwa, microwave synthesis inogona kushandiswa.Iyi nzira ine mhedzisiro inoshamisa, uye inoderedza zvakanyanya nguva yekuita, uye inoderedza kuumbwa kwemakiyi maviri ezvigadzirwa.
Fragment synthesis nzira inogona kushandiswa: Kana mamwe mapeptide achinetsa kugadzirwa nenzira dzakajairika uye zvisiri nyore kucheneswa, tinogona kutora kudzoreredzwa kweamino acid akati wandei mune chimwe chikamu chepeptide kune peptide cheni yese.Iyi nzira inogonawo kugadzirisa matambudziko akawanda mu synthesis.
Acylhydrazide synthesis inogona kushandiswa: acylhydrazide synthesis ye peptides inzira yakasimba-phase synthesis yeN-terminal Cys peptide uye C-terminal polypeptide hydrazide chemical selective reaction pakati pekugadzirwa kweamide bonds kuti uwane peptide bonding nzira.Zvichienderana nechinzvimbo cheCys mucheni yepeptide, iyi nzira inopatsanura peptide yese cheni kuita akawanda akatevedzana uye anoagadzira zvakateerana.Pakupedzisira, iyo peptide inonangwa inowanikwa kuburikidza nemvura-chikamu condensation reaction.Iyi nzira haingodzikisi zvakanyanya nguva yekubatanidza yepeptide refu, asi zvakare inowedzera zvakanyanya kuchena kwechigadzirwa chekupedzisira.
Kucheneswa kwepeptide refu
Hunhu hwemapeptide marefu zvinotungamira kune zvakaomarara zvikamu zve crude peptides.Naizvozvo, zvakare zvinonetsa kuchenesa refu peptides neHPLC.amyloid yakatevedzana yepolypeptide yekuchenesa maitiro, inotora yakawanda ruzivo uye yakabudirira kushandiswa mukucheneswa kwepeptide refu.Nekutora midziyo mitsva, kusanganiswa kwemaitiro akawanda ekuchenesa, kupatsanurwa kwakadzokororwa uye dzimwe nzira dzechiitiko, chiyero chekubudirira kwekucheneswa kwepeptide kwenguva refu chakavandudzwa zvakanyanya.
Nguva yekutumira: Kubvumbi-18-2023