Nzira itsva yekuita uye kugadzirira kwe thymectomy

Nzira itsva yeThymus inoumbwa ne28 amino acid synthetic polypeptide immune regulator.Thymus nzira imhando yemishonga mitsva yeuct, inowanzoshandiswa muvarwere vebundu, inogona kuwedzera kusadzivirirwa kwezvirwere.

Nzira yekuita

Iyo chaiyo nzira ye thymalfasin mukurapa kwekusingaperi hepatitis B haisati yanyatsodzidza, asi miedzo yakasiyana-siyana yakaratidza kuti thymalfasin ine simba rekudzivirira zvirwere.Iyo inonyanya kuitwa nekuvandudza T cell kushanda, uye inomhanya kuburikidza nematunhu anosanganisira T cell kugadzirwa, kupatsanura uye kunatswa, uye apoptosis.Nekuwedzera huwandu hwakakwana hwe lymphoid, intrahepatic natural killer, CD3 uye CD4 masero, thymalfasin inogona kutamba immunomodulatory basa.Pleural fasin inogona kukurudzira maturation uye kupatsanurwa kweDC masero, uye nekudaro inosimudzira kukura uye kupatsanurwa kweT masero.Thymalfasin inogona kumisa mitogen kana antigen, kuwedzera kugadzirwa kwemhando dzakasiyana-siyana dzema cytokines akadai se interferons human interleukin IL2 uye IL3, kuwedzera kutaura kwe interleukin-2 receptor, uye kuita kuti masero eCD4T asiyane muTHLS, ane akakosha antiviral mhedzisiro.Nekuwedzera basa reP masero, inogona kukurudzira kugadzirwa kwemalymphocytes mu vivo, kugadzirisa chikamu cheP cell subsets uye U cell kusiyanisa, pamwe nekugadzirwa kwemasoja ekudzivirira chirwere, kukurudzira kugadzirwa kwemamwe antiviral lymphokines uye macrophages, kuwedzera kumedza. shanda, uye kuvandudza mashandiro emasero immune system mune varwere vegomarara.Inogonawo kubvisa mabhakitiriya, mabhakitiriya uye mavhairasi kubva mumuviri.Bhuku reThymus nzira yenheyo system uye yakatenderedza endothelium, inosimuka kugadzirisa basa reiyo hippocampal neuron synaptic transmission.Thymus nzira itsva inonakidza synaptic kutapurirana uye tsinga sisitimu, inogona kushandiswa kudzivirira varwere vegomarara rinokonzerwa nekurapa cancer senge chemotherapy uye neurotoxicity.Pleuraxin ine gene regulatory mhedzisiro, inogona kukurudzira kinase cascade majini, kuwedzera cytokine majini, immune reaction regulatory genes.Iyo kumusoro-mutemo we thymalfasin paIL-senge receptor genes inoratidza kuti thymalfasin inomutsa maantigen arikuunza maseru."Uyezve, mhedzisiro ye thymalfasin pakukura kwemasero emuviri inoratidzwa mukuwedzera kwekutaura kwe stem cell kukura zvinhu uye hematopoietic mapuroteni majini."

Kutsva kutevedzana kwe thymectomy

Nzira yekugadzirira

1. Biochemical extraction method Thymosin preparations (musanganiswa we peptides) inowanikwa kubva kumhuka dzemhuka, saka basa racho rakasiyana zvikuru.Nekuti inotova neprotein yemhuka, pane mukana wepamusoro wezviratidzo zveallergic.Iyo biochemically inowanikwa nzira ye thymus kubva kumunhu embryonic thymus itsva, ine goho rakaderera zvikuru asi rakanaka kwazvo.Tichifunga nezvezvinhu zvishoma, mutengo wepamusoro, goho rakaderera, uye maitiro ekutora akaomarara., matambudziko makuru ekugadzirwa, tarisiro yebudiriro haina tarisiro.

2. Chemical synthesis Inowanikwa nekutengesa thymusfasin inonyanya kugadzirwa seSPSS, inogadzirwa nekucheneswa nehunyanzvi hwakakodzera.Zvichienderana neresin, Fmoc yakashandiswa kuumbwa kwakasimba kwechikamu, uye makumi maviri nemasere emhando dzeamino acids dzakagadzirwa zvinoenderana ne thymalfasin.N-terminal amino acids yaive acetylated neacetic anhydride, uye TFA yakashandiswa sechinhu chakakosha chekupwanya reagent yekupwanya kuwana rough zvigadzirwa.Chigadzirwa chisina kuchena chakaparadzaniswa neHPLC kuti iwane itsva thymus nzira chigadzirwa, iyo yakazocheneswa zvakare kuti iwane itsva thymus nzira mbishi.

Kune marudzi matatu emabhakitiriya akagadzirwa nemajini1) Majini akawanda anoumbwa mukati memuenzi kuti awane chinangwa chemajini, uye pakupedzisira anoratidzwa muchimiro chemiviri inosanganisirwa.Zvinonzi thymmethod novel akawanda-tambo majini akaiswa neasina kujairika annealing uye PCR matekiniki uye akadzokororwa muEscherichia coli.(2) Iyo yakagadzirwa fusion protein gene yakasanganiswa zvakare munhau, uye midhiya yakashandurwa kuita muenzi kuti ivake mabhakitiriya akagadzirwa.Semuenzaniso, zvichibva pachiratidzo chepeptide gene muplasmid, izwi rekuti plasmid rakavakwa, rakashandurwa kuita Pichia pastoris ne electroexcitation, uye ipapo puroteni yekubatanidza yakakonzerwa nemethanol, uyezve nzira itsva ye thymus yakagadzirwa ne enzyme digestion uye dzimwe nzira. .