Zvinoenderana nekubatanidza nzira yeamino acid uye shuga, shuga peptide inogona kukamurwa kuita zvikamu zvina: O glycosylation, C a N glycosylation, dew saccharification uye GPI (glycophosphatidlyinositol) kubatana.
1. N-glycosylation glycopeptides inoumbwa neN-acetamide glucose pakupera kwekudzikisa kweketani yeglycan (Glc-Nac) yakabatanidzwa neN atomu paboka reamide rechikamu chechikamu cheAsn muketani yepeptide, uye Asn. inokwanisa kubatanidza ketani yeglycan inofanira kunge iri muAsN-X-Ser / Thr (X! = P) mune motif inoumbwa nemasara.Shuga iN-acetylglucosamine.
N-glycosylation yakagadziridzwa structural glycopeptide
2. Chimiro cheO-glycosylation chiri nyore kupfuura N-glycosylation.Iyi glycopeptide kazhinji ipfupi pane glycan, asi ine marudzi akawanda kupfuura N-glycosylation.Ser uye Thr anogona kazhinji kuve glycosylated mu peptide cheni.Mukuwedzera, kune glycopeptides yakashongedzwa ne tyrosine, hydroxyl, uye hydroxyproline glycosylation.Nzvimbo yekubatanidza ndiyo hydroxyl okisijeni atomu padivi cheni yezvisaririra.Mashuga akabatanidzwa galactose kana N-acetylgalactosamine (Gal&GalNAc) kana glucose/glucosamine (Glc/GlcNAc), mannose/mannosamine (Man/ManNAc), nezvimwewo.
O-glycosylation inoshandura chimiro
3. Glycopeptide O-GlcNAC glycosylation ((N-acetylcysteine (NAC)) (glcnAcN-acetylglucosamine/acetylglucosamine)
Imwe N-acetylglucosamine (GlcNAc) glycosylation inobatanidza mapuroteni O-GlcNAc kune hydroxyl oxygen atomu ye serine kana threonine yakasara yeprotein.O-GlcNA glycosylation is O-GlcNAc monosaccharide chishongo pasina glycan kuwedzera;Kufanana ne peptide phosphorylation, O-GlcNAc glycosylation ye glycopeptides zvakare ine simba mapuroteni ekushongedza maitiro.Abnormal O-GlcNAc kushongedzwa kunogona kukonzera dzakasiyana siyana zvirwere zvakadai seshuga, chirwere chemwoyo, mapundu, chirwere cheAlzheimer uye zvichingodaro.
Glycosylation points ye glycopeptides
Izvo zvakakosha zvimiro zve polypeptide uye shuga cheni zvakabatanidzwa neprotein cheni nema covalent bond, uye masaiti anobatanidza shuga cheni anonzi glycosylation nzvimbo.Sezvo pasina template yekutevera biosynthesis ye glycopeptide shuga maketani, zvakasiyana-siyana zveshuga maketani achabatanidzwa kune imwechete glycosylation site, zvichitungamirira kune inonzi microscopic inhomogeneity.
Glycosylation ye glycopeptides
1. Mhedzisiro yeglycopeptide glycosylation pakurapa-kubudirira kwemapuroteni ekurapa.
Panyaya yekurapa-kurapa mapuroteni, glycosylation inokanganisawo hafu yehupenyu uye kunangwa kweprotein mishonga mu vivo.
2. Soluble glycopeptide glycosylation uye mapuroteni
Zvidzidzo zvakaratidza kuti maketani eshuga ari pamusoro pemapuroteni anogona kuvandudza mamolecular solubility yemapuroteni
3. Glycopeptide glycosylation uye protein immunogenicity
Kune rimwe divi, maketani eshuga ari pamusoro pemapuroteni anogona kuunza mhinduro chaiyo yekudzivirira.Kune rumwe rutivi, maketani eshuga anogona kuvhara mamwe mapeji pamusoro peprotein uye kuderedza immunogenicity yayo
4. Glycopeptide glycosylation inowedzera mapuroteni kugadzikana
Glycosylation inogona kuwedzera kugadzikana kwemapuroteni kune akasiyana denaturation mamiriro (senge denaturants, kupisa, nezvimwewo) uye kudzivirira kuunganidzwa kwemapuroteni.Panguva imwecheteyo, maketani eshuga ari pamusoro pemapuroteni anogona zvakare kuvhara mamwe maproteolytic degradation mapoinzi emaprotein mamorekuru, nekudaro achiwedzera kuramba kweprotein kune mapuroteni.
5. Glycopeptide glycosylation inokanganisa biological kuita kweprotein molecules
Kuchinja mapuroteni glycosylation kunogonawo kugonesa mapuroteni mamorekuru kuti aumbe zviitiko zvitsva zvebiology
Nguva yekutumira: Aug-03-2023