Dhizaini chirongwa uye mhinduro ye polypeptide peptide ketani

I. Pfupiso
Mapeptides akakosha macromolecules zvekuti kutevedzana kwawo hakuna kujairika mumakemikari uye maitiro emuviri.Mamwe mapeptide akaoma kubatanidza, nepo mamwe ari nyore kugadzira asi akaoma kuchenesa.Dambudziko rinoshanda nderekuti mapeptide mazhinji anonyungudika zvishoma mumigero ine aqueous, saka mukucheneswa kwedu, chikamu chinoenderana chehydrophobic peptide chinofanirwa kunyungudutswa mune zvisiri-aqueous solvents, Naizvozvo, izvi zvinonyungudutsa kana mabuffer zvinogona kunge zvisingaenderane zvakanyanya nekushandiswa. yebiological experimental procedures, kuitira kuti ma technicians arambidzwe zvachose kushandisa peptide nezvinangwa zvavo, zvekuti zvinotevera zvinhu zvakati wandei zvemagadzirirwo emapeptide evaongorori.

Dhizaini chirongwa uye mhinduro ye polypeptide peptide ketani
Chechipiri, sarudzo chaiyo yekugadzira yakaoma peptides
1. Hurefu hwese hwezvirongwa zvakaderera
Mapeptide asingasviki gumi neshanu asara ari nyore kuwana nekuti saizi yepeptide inowedzera uye kuchena kwechigadzirwa chisina kuchena kunodzikira.Sezvo hurefu hwese hwepeptide cheni hunowedzera kupfuura makumi maviri asara, chaihwo huwandu hwechigadzirwa chinhu chakakosha kunetseka.Mukuedza kwakawanda, zviri nyore kuwana zvisingatarisirwi mhedzisiro nekudzikisa yasara nhamba pazasi 20.
2. Kuderedza kuwanda kwema hydrophobic residues
Peptides ine huwandu hukuru hwema hydrophobic residues, kunyanya munharaunda 7-12 zvakasara kubva kuC-terminus, zvinowanzokonzera matambudziko ekugadzira.Izvi zvinoonekwa semusanganiswa usina kukwana chaizvo nekuti B-peta pepa rinowanikwa mumubatanidzwa."Mumamiriro ezvinhu akadai, zvingave zvinobatsira kushandura masara maviri akanaka uye asina kunaka, kana kuisa Gly kana Pro mupeptide kuvhura peptide."
3. Kuderedzwa kwe "zvakaoma" zvakasara
"Kune akati wandei eCys, Met, Arg, uye Edza zvisaririra izvo zvisingawanzo kugadzirwa."Ser inowanzo shandiswa senge nonoxidative imwe nzira kune Cys.
Dhizaini chirongwa uye mhinduro ye polypeptide peptide ketani

Chechitatu, kunatsiridza sarudzo chaiyo yekunyungudika mumvura
1. Gadzirisa N kana C terminus
Zvinei kune acidic peptides (kureva kuti, yakashata yakachajwa papH 7), acetylation (N-terminus acetylation, C terminus inogara ichichengeta yemahara carboxyl boka) inonyanya kukurudzirwa kuwedzera iyo yakaipa charge.Nekudaro, kune mapeptides ekutanga (kureva kuti, akachajwa zvakanaka papH 7), amination (yemahara amino boka paN-terminus uye amination paC-terminus) inonyanya kukurudzirwa kuwedzera mutero wakanaka.

2. Kupfupisa kana kurebesa kutevedzana

Zvimwe zvezvirongwa zvine nhamba huru ye hydrophobic amino acids, yakadai seTrp, Phe, Val, Ile, Leu, Met, Tyr uye Ala, etc. Apo aya masara e-hydrophobic anopfuura 50%, kazhinji haasi nyore kuparara.Zvinogona kubatsira kurebesa kutevedzana kuti uwedzere kuwedzera matanda akanaka uye asina kunaka epeptide.Chechipiri sarudzo ndeyekudzikisira saizi yeketani yepeptide kuti uwedzere matanda akanaka uye asina kunaka nekudzikisira masara ehydrophobic.Iyo yakasimba iyo yakanaka uye yakaipa mativi echeni yepeptide, zvakanyanya mukana wekuita nemvura.
3. Isa mumvura inonyungudika yakasara
Kune mamwe macheni epeptide, musanganiswa wemamwe akanaka uye asina kunaka amino acids anogona kuvandudza kunyungudika kwemvura.Kambani yedu inokurudzira iyo N-terminus kana C-terminus ye acidic peptides kuti ibatanidzwe neGlu-Glu.Iyo N kana C terminus yeyakakosha peptide yakapihwa uyezve Lys-Ls.Kana boka rinopomerwa risingakwanisi kuiswa, Ser-Gly-Ser inogonawo kuiswa muN kana C terminus.Nekudaro, iyi nzira haishande kana mativi echeni yepeptide isingashandurwe.