HIV-1 tat Mapuroteni (49-57)/123251-89-8 /GT Peptide/Peptide Supplier

Tsanangudzo 

Maprotein eHIV-1 tat (49-57) ari mukati meHIV-1 tat protein transduction domain (PTD) uye anokwana kufambisa mapuroteni ane heterologous kuyambuka membrane yeplasma.Kuti iwedzere kunyungudika, chubhu yakadziya kusvika ku37° C uye yakazununguswa mubhati re ultrasonic kwenguva yakati.Iyo stock solution inogona kuchengetwa pazasi -20° C kwemwedzi inoverengeka.HIV-1 tat Mapuroteni (49-57)iriwo mushonga wepeptide

Zvinotsanangurwa

Apperance: Ichena kusvika kune-chena poda

Kuchena(HPLC):≥98.0%

Kusachena Kumwechete:≤2.0%

Acetate Content (HPLC): 5.0%～12.0%

Mvura Yemukati (Karl Fischer):≤10.0%

Peptide Content:≥80.0%

Kurongedza uye Kutumira: Kudziya kwakadzika, vacuum kurongedza, kwakaringana kumg sezvinodiwa.

Kuhodha sei?

1. Contact us directly by phone or email: +86-13735575465, sales1@gotopbio.com.

2. Order online.Ndapota zadza odha yepamhepo fomu.

3. Ipa peptide zita, CAS No. kana kutevedzana, kuchena uye kugadziriswa kana zvichidiwa, uwandu, nezvimwewo tichapa quotation mukati maawa maviri.

4. Order conformation nechibvumirano chekutengesa chakasainwa uye NDA (chibvumirano chisina kuburitswa) kana chibvumirano chakavanzika.

5. Ticharamba tichivandudza kufambira mberi kwehurongwa munguva.

6. Peptide kuendeswa neDHL, Fedex kana vamwe, uye HPLC, MS, COA ichagoverwa pamwe chete nekutakura.

7. Refund policy ichateverwa kana paine kupesana kwehunhu hwedu kana sevhisi.

8. Mushure mekutengesa sevhisi: Kana vatengi vedu vaine mibvunzo nezve peptide yedu panguva yekuyedza, ndapota inzwa wakasununguka kutibata nesu uye isu tichaipindura kwairi munguva pfupi.

Zvese zvigadzirwa zvekambani zvinongo shandiswa sesainzi yekutsvaga chinangwa, iyo'inorambidzwa kushandiswa zvakananga nemunhu chero upi zvake pamuviri wemunhu.

FAQ:

Mapeptides aive neCys akaderedzwa here asati atumirwa?

Kana iyo peptide ikasawanikwa yakave neoxidized, isu kazhinji hatideredze maCys.Ese ma polypeptides anowanikwa kubva kune zvisina kucheneswa zvigadzirwa zvakacheneswa uye lyophilized pasi pepH2 mamiriro, ayo anosvika pamwero wakati kudzivirira oxidation yeCys.Peptides ine Cys inocheneswa pa pH2 kunze kwekunge paine chikonzero chaicho chekuchenesa papH6.8.Kana kucheneswa kuchiitwa pa pH6.8, chigadzirwa chakacheneswa chinofanira kurapwa neasidhi nekukurumidza kudzivirira oxidation.Muchikamu chekupedzisira chekutonga kwemhando, kune peptides ine Cys, kana kuvepo kwehuremu hwemamorekuru (2P + H) chinhu chinowanikwa pamepu yeMS, inoratidza kuti dimer yakaumbwa.Kana pasina dambudziko neMS uye HPLC, isu tichaophilize zvakananga uye nekutumira zvinhu pasina kumwe kugadzirisa.Izvo zvinofanirwa kucherechedzwa kuti peptides ine Cys inononoka oxidation nekufamba kwenguva, uye dhigirii reoxidation zvinoenderana nekutevedzana kwepeptide uye kuchengetedza mamiriro.

Unoziva sei kana peptide yakasungwa?

Isu tinoshandisa Ellman reaction kuti tiedze kana kuumbwa kwemhete kwapera.Kana bvunzo yeEllman iri positive (yero), kuita kwemhete hakuna kukwana.Kana bvunzo dzabuda dzisina (kwete yero), kuita kweringin kwapera.Isu hatipe iyo yekuongorora rondedzero yekuzivikanwa kwe cyclization kune vatengi vedu.Kazhinji, pachave nerondedzero yeEllman's test results in the QC report.

Ini ndinoda cyclic peptide, ine tryptophan, ichave yakasvibiswa here?

Iyo oxidation ye tryptophan chinhu chakajairika mu peptide oxidation, uye peptides anowanzo cycled pamberi pekucheneswa.Kana iyo oxidation ye tryptophan ikaitika, nguva yekuchengeta peptide paHPLC column ichachinja, uye oxidation inogona kubviswa nekucheneswa.Uyezve, oxidized peptides inogonawo kuonekwa neMS.

Zvakakosha here kuisa mukaha pakati pepeptide nedhayi?

Kana uchizobatanidza molekuru hombe (sedhayi) kune peptide, zviri nani kuisa nzvimbo pakati pepeptide ne ligand kuti uderedze kupindirana neanogamuchira nekupeta peptide pachayo kana nekupeta kwe. kusangana kwayo.Vamwe havadi ma intervals.Semuyenzaniso, mukupeta mapuroteni, zvinokwanisika kuona kuti kureba kwakadii kupeta chimiro cheamino acid nekubatanidza dhayi yefluorescent kune imwe saiti.

Kana iwe uchida kuita biotin gadziriso paN terminal, unofanirwa kuisa mukaha pakati pebiotin uye peptide sequence?

Iyo yakajairwa biotin labeling maitiro anoshandiswa nekambani yedu kubatanidza Ahx kune peptide cheni, ichiteverwa nebiotin.Ahx i6-carbon compound iyo inoshanda sechivharo pakati pepeptide nebiotin.

Iwe unogona here kupa rimwe zano pamusoro pekugadzirwa kwephosphorylated peptides?

Sezvo kureba kunowedzera, kushanda kwekubatanidza kunoderera zvishoma nezvishoma kubva kune phosphorylated amino acid zvichienda mberi.Iyo nzira yekubatanidza inobva kuC terminal kuenda kuN terminal.Zvinokurudzirwa kuti zvakasara mushure me phosphorylated amino acid haifanire kudarika gumi, kureva kuti, nhamba yemamino acid inosara isati yasvika phosphorylated amino acid kubva kuN terminal kuenda kuC terminal haifanirwe kudarika gumi.

Sei iyo n-terminal acetylation uye C-terminal amidation?

Aya magadzirirwo anodzivirira peptide kubva pakuderedzwa uye inobvumira peptide kutevedzera mamiriro ayo ekutanga ealpha amino nemapoka ecarboxyl muprotein yevabereki.